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RBM28 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM28 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128282-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM28 (NM_001166135), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBM28 | RNA binding motif protein 28 | RNA-binding motif protein 28 | RNA-binding protein 28
Origin Species
Human
NCBI GenBank
NM_018077
SwissProt
Q9NW13

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLTLFVGR LPPSARSEQL EELFSQVGPV KQCFVVTEKG RTVAVDWAVA KDKYKDTQSV
61SAIGEEKSHE SKHQESVKKK GREEEDMEEE ENDDDDDDDD EEDGVFDDED EEEENIESKV
121TKPVQIQKRA VKRPAPAKSS DHSEEDSDLE ESDSIDDGEE LAQSDTSTEE QEDKAVQVSN
181KKKRKLPSDV NEGKTVFIRN LSFDSEEEEL GELLQQFGEL KYVRIVLHPD TEHSKGCAFA
241QFMTQEAAQK CLLAASPENE AGGLKLDGRQ LKVDLAVTRD EAAKLQTTKV KKPTGTRNLY
301LAREGLIRAG TKAAEGVSAA DMAKRERFEL LKHQKLKDQN IFVSRTRLCL HNLPKAVDDK
361QLRKLLLSAT SGEKGVRIKE CRVMRDLKGV HGNMKGQSLG YAFAEFQEHE HALKALRLIN
421NNPEIFGPLK RPIVEFSLED RRKLKMKELR IQRSLQKMRS KPATGEPQKG QPEPAKDQQQ
481KAAQHHTEEQ SKVPPEQKRK AGSTSWTGFQ TKAEVEQVEL PDGKKRRKVL ALPSHRGPKI
541RLRDKGKVKP VHPKKPKPQI NQWKQEKQQL SSEQVSRKKA KGNKTETRFN QLVEQYKQKL
601LGPSKGAPLA KRSKWFDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

618 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.