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RBM27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM27 antibodies →
$725.00each
Catalog Number: LS-G127145-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM27 (NM_018989), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1311 | Psc1 | RBM27 | RNA-binding motif protein 27 | RNA-binding protein 27 | ARRS1 | RNA binding motif protein 27
Origin Species
Human
NCBI GenBank
AB037732
SwissProt
Q9P2N5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
118.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLIEDVDALK SWLAKLLEPI CDADPSALAN YVVALVKKDK PEKELKAFCA DQLDVFLQKE
61TSGFVDKLFE SLYTKNYLPL LEPVKPEPKP LVQEKEEIKE EVFQEPAEEE RDGRKKKYPS
121PQKTRSESSE RRTREKKRED GKWRDYDRYY ERNELYREKY DWRRGRSKSR SKSRGLSRSR
181SRSRGRSKDR DPNRNVEHRE RSKFKSERND LESSYVPVSA PPPNSSEQYS SGAQSIPSTV
241TVIAPAHHSE NTTESWSNYY NNHSSSNSFG RNLPPKRRCR DYDERGFCVL GDLCQFDHGN
301DPLVVDEVAL PSMIPFPPPP PGLPPPPPPG MLMPPMPGPG PGPGPGPGPG PGPGPGPGHS
361MRLPVPQGHG QPPPSVVLPI PRPPITQSSL INSRDQPGTS AVPNLASVGT RLPPPLPQNL
421LYTVSERQPM YSREHGAAAS ERLQLGTPPP LLAARLVPPR NLMGSSIGYH TSVSSPTPLV
481PDTYEPDGYN PEAPSITSSG RSQYRQFFSR TQTQRPNLIG LTSGDMDVNP RAANIVIQTE
541PPVPVSINSN ITRVVLEPDS RKRAMSGLEG PLTKKPWLGK QGNNNQNKPG FLRKNQYTNT
601KLEVKKIPQE LNNITKLNEH FSKFGTIVNI QVAFKGDPEA ALIQYLTNEE ARKAISSTEA
661VLNNRFIRVL WHRENNEQPT LQSSAQLLLQ QQQTLSHLSQ QHHHLPQHLH QQQVLVAQSA
721PSTVHGGIQK MMSKPQTSGA YVLNKVPVKH RLGHAGGNQS DASHLLNQSG GAGEDCQIFS
781TPGHPKMIYS SSNLKTPSKL CSGSKSHDVQ EVLKKKQEAM KLQQDMRKKR QEVLEKQIEC
841QKMLISKLEK NKNMKPEERA NIMKTLKELG EKISQLKDEL KTSSAVSTPS KVKTKTEAQK
901ELLDTELDLH KRLSSGEDTT ELRKKLSQLQ VEAARLGILP VGRGKTMSSQ GRGRGRGRGG
961RGRGSLNHMV VDHRPKALTV GGFIEEEKED LLQHFSTANQ GPKFKDRRLQ ISWHKPKVPS
1021ISTETEEEEV KEEETETSDL FLPDDDDEDE DEYESRSWRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1060 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.