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RBM20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM20 antibodies →
$725.00each
Catalog Number: LS-G125241-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM20 (NM_001134363), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBM20 | RNA-binding motif protein 20 | RNA binding motif protein 20
Origin Species
Human
SwissProt
Q5T481

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLAAAMSQD ADPSGPEQPD RVACSVPGAR ASPAPSGPRG MQQPPPPPQP PPPPQAGLPQ
61IIQNAAKLLD KNPFSVSNPN PLLPSPASLQ LAQLQAQLTL HRLKLAQTAV TNNTAAATVL
121NQVLSKVAMS QPLFNQLRHP SVITGPHGHA GVPQHAAAIP STRFPSNAIA FSPPSQTRGP
181GPSMNLPNQP PSAMVMHPFT GVMPQTPGQP AVILGIGKTG PAPATAGFYE YGKASSGQTY
241GPETDGQPGF LPSSASTSGS VTYEGHYSHT GQDGQAAFSK DFYGPNSQGS HVASGFPAEQ
301AGGLKSEVGP LLQGTNSQWE SPHGFSGQSK PDLTAGPMWP PPHNQPYELY DPEEPTSDRT
361PPSFGGRLNN SKQGFIGAGR RAKEDQALLS VRPLQAHELN DFHGVAPLHL PHICSICDKK
421VFDLKDWELH VKGKLHAQKC LVFSENAGIR CILGSAEGTL CASPNSTAVY NPAGNEDYAS
481NLGTSYVPIP ARSFTQSSPT FPLASVGTTF AQRKGAGRVV HICNLPEGSC TENDVINLGL
541PFGKVTNYIL MKSTNQAFLE MAYTEAAQAM VQYYQEKSAV INGEKLLIRM SKRYKELQLK
601KPGKAVAAII QDIHSQRERD MFREADRYGP ERPRSRSPVS RSLSPRSHTP SFTSCSSSHS
661PPGPSRADWG NGRDSWEHSP YARREEERDP APWRDNGDDK RDRMDPWAHD RKHHPRQLDK
721AELDERPEGG RPHREKYPRS GSPNLPHSVS SYKSREDGYY RKEPKAKWDK YLKQQQDAPG
781RSRRKDEARL RESRHPHPDD SGKEDGLGPK VTRAPEGAKA KQNEKNKTKR TDRDQEGADD
841RKENTMAENE AGKEEQEGME ESPQSVGRQE KEAEFSDPEN TRTKKEQDWE SESEAEGESW
901YPTNMEELVT VDEVGEEEDF IVEPDIPELE EIVPIDQKDK ICPETCLCVT TTLDLDLAQD
961FPKEGVKAVG NGAAEISLKS PRELPSASTS CPSDMDVEMP GLNLDAERKP AESETGLSLE
1021DSDCYEKEAK GVESSDVHPA PTVQQMSSPK PAEERARQPS PFVDDCKTRG TPEDGACEGS
1081PLEEKASPPI ETDLQNQACQ EVLTPENSRY VEMKSLEVRS PEYTEVELKQ PLSLPSWEPE
1141DVFSELSIPL GVEFVVPRTG FYCKLCGLFY TSEETAKMSH CRSAVHYRNL QKYLSQLAEE
1201GLKETEGADS PRPEDSGIVP RFERKKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1227 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.