LSBio
RBM12B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM12B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM12B antibodies →
$725.00each
Catalog Number: LS-G103110-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM12B (NM_203390), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MGC:33837 | RBM12B | RNA binding motif protein 12B | RNA-binding motif protein 12B | RNA-binding protein 12B
Origin Species
Human
NCBI GenBank
NM_203390
SwissProt
Q8IXT5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVVIRLLGL PFIAGPVDIR HFFTGLTIPD GGVHIIGGEI GEAFIIFATD EDARRAISRS
61GGFIKDSSVE LFLSSKAEMQ KTIEMKRTDR VGRGRPGSGT SGVDSLSNFI ESVKEEASNS
121GYGSSINQDA GFHTNGTGHG NLRPRKTRPL KAENPYLFLR GLPYLVNEDD VRVFFSGLCV
181DGVIFLKHHD GRNNGDAIVK FASCVDASGG LKCHRSFMGS RFIEVMQGSE QQWIEFGGNA
241VKEGDVLRRS EEHSPPRGIN DRHFRKRSHS KSPRRTRSRS PLGFYVHLKN LSLSIDERDL
301RNFFRGTDLT DEQIRFLYKD ENRTRYAFVM FKTLKDYNTA LSLHKTVLQY RPVHIDPISR
361KQMLKFIARY EKKRSGSLER DRPGHVSQKY SQEGNSGQKL CIYIRNFPFD VTKVEVQKFF
421ADFLLAEDDI YLLYDDKGVG LGEALVKFKS EEQAMKAERL NRRRFLGTEV LLRLISEAQI
481QEFGVNFSVM SSEKMQARSQ SRERGDHSHL FDSKDPPIYS VGAFENFRHQ LEDLRQLDNF
541KHPQRDFRQP DRHPPEDFRH SSEDFRFPPE DFRHSPEDFR RPREEDFRRP SEEDFRRPWE
601EDFRRPPEDD FRHPREEDWR RPLEEDWRRP LEEDFRRSPT EDFRQLPEED FRQPPEEDLR
661WLPEEDFRRP PEEDWRRPPE EDFRRPLQGE WRRPPEDDFR RPPEEDFRHS PEEDFRQSPQ
721EHFRRPPQEH FRRPPPEHFR RPPPEHFRRP PPEHFRRPPP EHFRRPPPEH FRRPPPEHFR
781RPPQEHFRRP PQEHFRRSRE EDFRHPPDED FRGPPDEDFR HPPDEDFRSP QEEDFRCPSD
841EDFRQLPEED LREAPEEDPR LPDNFRPPGE DFRSPPDDFR SHRPFVNFGR PEGGKFDFGK
901HNMGSFPEGR FMPDPKINCG SGRVTPIKIM NLPFKANVNE ILDFFHGYRI IPDSVSIQYN
961EQGLPTGEAI VAMINYNEAM AAIKDLNDRP VGPRKVKLTL L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1001 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.