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RBM12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBM12 antibodies →
$725.00each
Catalog Number: LS-G115563-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM12 (NM_006047), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HRIHFB2091 | KIAA0765 | RBM12 | RNA-binding protein 12 | RNA-binding motif protein 12 | SWAN | RNA binding motif protein 12
Origin Species
Human
NCBI GenBank
NM_006047
SwissProt
Q9NTZ6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAVVIRLQGL PIVAGTMDIR HFFSGLTIPD GGVHIVGGEL GEAFIVFATD EDARLGMMRT
61GGTIKGSKVT LLLSSKTEMQ NMIELSRRRF ETANLDIPPA NASRSGPPPS SGMSSRVNLP
121TTVSNFNNPS PSVVTATTSV HESNKNIQTF STASVGTAPP NMGASFGSPT FSSTVPSTAS
181PMNTVPPPPI PPIPAMPSLP PMPSIPPIPV PPPVPTLPPV PPVPPIPPVP SVPPMTPLPP
241MSGMPPLNPP PVAPLPAGMN GSGAPMNLNN NLNPMFLGPL NPVNPIQMNS QSSVKPLPIN
301PDDLYVSVHG MPFSAMENDV RDFFHGLRVD AVHLLKDHVG RNNGNGLVKF LSPQDTFEAL
361KRNRMLMIQR YVEVSPATER QWVAAGGHIT FKQNMGPSGQ THPPPQTLPR SKSPSGQKRS
421RSRSPHEAGF CVYLKGLPFE AENKHVIDFF KKLDIVEDSI YIAYGPNGKA TGEGFVEFRN
481EADYKAALCR HKQYMGNRFI QVHPITKKGM LEKIDMIRKR LQNFSYDQRE MILNPEGDVN
541SAKVCAHITN IPFSITKMDV LQFLEGIPVD ENAVHVLVDN NGQGLGQALV QFKNEDDARK
601SERLHRKKLN GREAFVHVVT LEDMREIEKN PPAQGKKGLK MPVPGNPAVP GMPNAGLPGV
661GLPSAGLPGA GLPSTGLPGS AITSAGLPGA GMPSAGIPSA GGEEHAFLTV GSKEANNGPP
721FNFPGNFGGS NAFGPPIPPP GLGGGAFGDA RPGMPSVGNS GLPGLGLDVP GFGGGPNNLS
781GPSGFGGGPQ NFGNGPGSLG GPPGFGSGPP GLGSAPGHLG GPPAFGPGPG PGPGPGPIHI
841GGPPGFASSS GKPGPTVIKV QNMPFTVSID EILDFFYGYQ VIPGSVCLKY NEKGMPTGEA
901MVAFESRDEA TAAVIDLNDR PIGSRKVKLV LG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

932 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.