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RBCK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBCK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBCK1 antibodies →
$725.00each
Catalog Number: LS-G115290-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBCK1 (NM_006462), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf18 | HBV-associated factor 4 | HOIL-1 | RBCK1 | RING finger protein 54 | UBCE7IP3 | ZRANB4 | RNF54 | XAP4 | HBV associated factor 4 | HOIL1 | RBCK2 | XAP3
Origin Species
Human
NCBI GenBank
NM_006462
SwissProt
Q9BYM8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTATPDGRE DQERLWVSVE DAQMHTVTIW LTVRPDMTVA SLKDMVFLDY GFPPVLQQWV
61IGQRLARDQE TLHSHGVRQN GDSAYLYLLS ARNTSLNPQE LQRERQLRML EDLGFKDLTL
121QPRGPLEPGP PKPGVPQEPG RGQPDAVPEP PPVGWQCPGC TFINKPTRPG CEMCCRARPE
181AYQVPASYQP DEEERARLAG EEEALRQYQQ RKQQQQEGNY LQHVQLDQRS LVLNTEPAEC
241PVCYSVLAPG EAVVLRECLH TFCRECLQGT IRNSQEAEVS CPFIDNTYSC SGKLLEREIK
301ALLTPEDYQR FLDLGISIAE NRSAFSYHCK TPDCKGWCFF EDDVNEFTCP VCFHVNCLLC
361KAIHEQMNCK EYQEDLALRA QNDVAARQTT EMLKVMLQQG EAMRCPQCQI VVQKKDGCDW
421IRCTVCHTEI CWVTKGPRWG PGGPGDTSGG CRCRVNGIPC HPSCQNCH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

468 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.