Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RBBP7 / RbAp46 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130975-100
Catalog Number: LS-G130975
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RbAp46(RBBP7) (NM_001198719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RbAp46 | RBBP-7 | Histone-binding protein RBBP7 | RBBP7
- Origin Species
- Human
- NCBI GenBank
- NM_002893
- SwissProt
- Q16576
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAEAGVVGA GASPDGDWRD QACGLLLHVH LSSRLGRAAP VRTGRHLRTV FEDTVEERVI61NEEYKIWKKN TPFLYDLVMT HALQWPSLTV QWLPEVTKPE GKDYALHWLV LGTHTSDEQN121HLVVARVHIP NDDAQFDASH CDSDKGEFGG FGSVTGKIEC EIKINHEGEV NRARYMPQNP181HIIATKTPSS DVLVFDYTKH PAKPDPSGEC NPDLRLRGHQ KEGYGLSWNS NLSGHLLSAS241DDHTVCLWDI NAGPKEGKIV DAKAIFTGHS AVVEDVAWHL LHESLFGSVA DDQKLMIWDT301RSNTTSKPSH LVDAHTAEVN CLSFNPYSEF ILATGSADKT VALWDLRNLK LKLHTFESHK361DEIFQVHWSP HNETILASSG TDRRLNVWDL SKIGEEQSAE DAEDGPPELL FIHGGHTAKI421SDFSWNPNEP WVICSVSEDN IMQIWQMAEN IYNDEESDVT TSELEGQGSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
469 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.