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RAVER1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAVER1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RAVER1 antibodies →
$725.00each
Catalog Number: LS-G107499-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAVER1 (NM_133452), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1978 | RAVER1 | Protein raver-1
Origin Species
Human
NCBI GenBank
NM_133452
SwissProt
Q8IY67

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLRPGSLVP EGAGFPKMAA DVSVTHRPPL SPKSGAEVEA GDAAERRAPE EELPPLDPEE
61IRKRLEHTER QFRNRRKILI RGLPGDVTNQ EVHDLLSDYE LKYCFVDKYK GTAFVTLLNG
121EQAEAAINAF HQSRLREREL SVQLQPTDAL LCVANLPPSL TQQQFEELVR PFGSLERCFL
181VYSERTGQSK GYGFAEYMKK DSAARAKSDL LGKPLGPRTL YVHWTDAGQL TPALLHSRCL
241CVDRLPPGFN DVDALCRALS AVHSPTFCQL ACGQDGQLKG FAVLEYETAE MAEEAQQQAD
301GLSLGGSHLR VSFCAPGPPG RSMLAALIAA QATALNRGKG LLPEPNILQL LNNLGPSASL
361QLLLNPLLHG SAGGKQGLLG APPAMPLLNG PALSTALLQL ALQTQGQKKP GILGDSPLGA
421LQPGAQPANP LLGELPAGGG LPPELPPRRG KPPPLLPSVL GPAGGDREAL GLGPPAAQLT
481PPPAPVGLRG SGLRGLQKDS GPLPTPPGVS LLGEPPKDYR IPLNPYLNLH SLLPASNLAG
541KEARGWGGAG RSRRPAEGPP TNPPAPGGGS SSSKAFQLKS RLLSPLSSAR LPPEPGLSDS
601YSFDYPSDMG PRRLFSHPRE PALGPHGPSR HKMSPPPSGF GERSSGGSGG GPLSHFYSGS
661PTSYFTSGLQ AGLKQSHLSK AIGSSPLGSG EGLLGLSPGP NGHSHLLKTP LGGQKRSFAH
721LLPSPEPSPE GSYVGQHSQG LGGHYADSYL KRKRIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

756 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.