LSBio
Rat GRN / Granulin Protein (Recombinant DDK (Flag))

Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.

Rat GRN / Granulin Protein (Recombinant DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
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$337.00each
Catalog Number: LS-G3864-10

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Overview

Description
Signal peptide and rat progranulin (aa 1-602) are fused at the C-terminus to a FLAG(R)-tag.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
Acrogranin | CLN11 | Epithelin precursor | GEP | GRN | gp88 | Granulin | Granulin-epithelin | Granulins | PC cell-derived growth factor | PCDGF | PEPI | PGRN | Proepithelin | Progranulin
Origin Species
Rat
NCBI GenBank
NM_002087|NP_002078.1
SwissProt
P28799

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
~65kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1aa 1-602

8 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
< 0.1 EU/µg
Format
Lyophilized
Formulation
Lyophilized from PBS
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store lyophilized at -20°C for up to 1 year. Once reconstituted store at 4°C for immediate use.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.