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RASSF10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RASSF10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119679-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASSF10 (NM_001080521), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RASSF10
Origin Species
Human
SwissProt
A6NK89

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPSEKKISV WICQEEKLVS GLSRRTTCSD VVRVLLEDGC RRRRRQRRSR RLGSAGDPHG
61PGELPEPPNE DDEDDDEALP QGMLCGPPQC YCIVEKWRGF ERILPNKTRI LRLWAAWGEE
121QENVRFVLVR SEASLPNAGP RSAEARVVLS RERPCPARGA PARPSLAMTQ EKQRRVVRKA
181FRKLAKLNRR RQQQTPSSCS STSSSTASSC SSSPRTHESA SVERMETLVH LVLSQDHTIR
241QQVQRLHELD REIDHYEAKV HLDRMRRHGV NYVQDTYLVG AGIELDGSRP GEEPEEVAAE
301AEEAAAAPPL AGEAQAAALE ELARRCDDLL RLQEQRVQQE ELLERLSAEI QEELNQRWMR
361RRQEELAARE EPLEPDGGPD GELLLEQERV RTQLSTSLYI GLRLNTDLEA VKSDLDYSQQ
421QWDSKKRELQ GLLQTLHTLE LTVAPDGAPG SGSPSREPGP QACADMWVDQ ARGLAKSGPG
481NDEDSDTGLS SMHSQDSDSL PMCESLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.