Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RASGRP3 / GRP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102408-100
Catalog Number: LS-G102408
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASGRP3 (NM_170672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRP3 | KIAA0846 | Ras guanyl-releasing protein 3 | RASGRP3
- Origin Species
- Human
- NCBI GenBank
- BC066127
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSSGLGKAA TLDELLCTCI EMFDDNGELD NSYLPRIVLL MHRWYLSSTE LAEKLLCMYR61NATGESCNEF RLKICYFMRY WILKFPAEFN LDLGLIRMTE EFREVASQLG YEKHVSLIDI121SSIPSYDWMR RVTQRKKVSK KGKACLLFDH LEPIELAEHL TFLEHKSFRR ISFTDYQSYV181IHGCLENNPT LERSIALFNG ISKWVQLMVL SKPTPQQRAE VITKFINVAK KLLQLKNFNT241LMAVVGGLSH SSISRLKETH SHLSSEVTKN WNEMTELVSS NGNYCNYRKA FADCDGFKIP301ILGVHLKDLI AVHVIFPDWT EENKVNIVKM HQLSVTLSEL VSLQNASHHL EPNMDLINLL361TLSLDLYHTE DDIYKLSLVL EPRNSKSQPT SPTTPNKPVV PLEWALGVMP KPDPTVINKH421IRKLVESVFR NYDHDHDGYI SQEDFESIAA NFPFLDSFCV LDKDQDGLIS KDEMMAYFLR481AKSQLHCKMG PGFIHNFQEM TYLKPTFCEH CAGFLWGIIK QGYKCKDCGA NCHKQCKDLL541VLACRRFARA PSLSSGHGSL PGSPSLPPAQ DEVFEFPGVT AGHRDLDSRA ITLVTGSSRK601ISVRLQRATT SQATQTEPVW SEAGWGDSGS HTFPKMKSKF HDKAAKDKGF AKWENEKPRV661HAGVDVVDRG TEFELDQDEG EETRQDGEDGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
690 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.