Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RASGRF2 / RAS-GRF2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114994-100
Catalog Number: LS-G114994
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASGRF2 (NM_006909), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRF2 | RAS-GRF2 | RASGRF2
- Origin Species
- Human
- NCBI GenBank
- AF023130
- SwissProt
- O14827
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 140.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQKSVRYNEG HALYLAFLAR KEGTKRGFLS KKTAEASRWH EKWFALYQNV LFYFEGEQSC61RPAGMYLLEG CSCERTPAPP RAGAGQGGVR DALDKQYYFT VLFGHEGQKP LELRCEEEQD121GKEWMEAIHQ ASYADILIER EVLMQKYIHL VQIVETEKIA ANQLRHQLED QDTEIERLKS181EIIALNKTKE RMRPYQSNQE DEDPDIKKIK KVQSFMRGWL CRRKWKTIVQ DYICSPHAES241MRKRNQIVFT MVEAESEYVH QLYILVNGFL RPLRMAASSK KPPISHDDVS SIFLNSETIM301FLHEIFHQGL KARIANWPTL ILADLFDILL PMLNIYQEFV RNHQYSLQVL ANCKQNRDFD361KLLKQYEANP ACEGRMLETF LTYPMFQIPR YIITLHELLA HTPHEHVERK SLEFAKSKLE421ELSRVMHDEV SDTENIRKNL AIERMIVEGC DILLDTSQTF IRQGSLIQVP SVERGKLSKV481RLGSLSLKKE GERQCFLFTK HFLICTRSSG GKLHLLKTGG VLSLIDCTLI EEPDASDDDS541KGSGQVFGHL DFKIVVEPPD AAAFTVVLLA PSRQEKAAWM SDISQCVDNI RCNGLMTIVF601EENSKVTVPH MIKSDARLHK DDTDICFSKT LNSCKVPQIR YASVERLLER LTDLRFLSID661FLNTFLHTYR IFTTAAVVLG KLSDIYKRPF TSIPVRSLEL FFATSQNNRG EHLVDGKSPR721LCRKFSSPPP LAVSRTSSPV RARKLSLTSP LNSKIGALDL TTSSSPTTTT QSPAASPPPH781TGQIPLDLSR GLSSPEQSPG TVEENVDNPR VDLCNKLKRS IQKAVLESAP ADRAGVESSP841AADTTELSPC RSPSTPRHLR YRQPGGQTAD NAHCSVSPAS AFAIATAAAG HGSPPGFNNT901ERTCDKEFII RRTATNRVLN VLRHWVSKHA QDFELNNELK MNVLNLLEEV LRDPDLLPQE961RKAAANILRA LSQDDQDDIH LKLEDIIQMT DCMKAECFES LSAMELAEQI TLLDHVIFRS1021IPYEEFLGQG WMKLDKNERT PYIMKTSQHF NDMSNLVASQ IMNYADVSSR ANAIEKWVAV1081ADICRCLHNY NGVLEITSAL NRSAIYRLKK TWAKVSKQTK ALMDKLQKTV SSEGRFKNLR1141ETLKNCNPPA VPYLGMYLTD LAFIEEGTPN FTEEGLVNFS KMRMISHIIR EIRQFQQTSY1201RIDHQPKVAQ YLLDKDLIID EDTLYELSLK IEPRLPATRRLEQKLISEEDLAANDILDYKDDDDKV
1240 aa + 26 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.