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RASEF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RASEF Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106169-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASEF (NM_152573), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RASEF | RAB45 | Ras-related protein Rab-45
Origin Species
Human
NCBI GenBank
AI337241

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEADGDGEEL ARLRSVFAAC DANRSGRLER EEFRALCTEL RVRPADAEAV FQRLDADRDG
61AITFQEFARG FLGSLRGGRR RDWGPLDPAP AVSEAGPETH DSEEDEGDED AAAALATSCG
121PASPGRAWQD FQARLGDEAK FIPREEQVST LYQNINLVEP RLIQPYEHVI KNFIREIRLQ
181STEMENLAIA VKRAQDKAAM QLSELEEEMD QRIQAAEHKT RKDEKRKAEE ALSDLRRQYE
241TEVGDLQVTI KKLRKLEEQS KRVSQKEDVA ALKKQIYDLS MENQKVKKDL LEAQTNIAFL
301QSELDALKSD YADQSLNTER DLEIIRAYTE DRNSLERQIE ILQTANRKLH DSNDGLRSAL
361ENSYSKFNRS LHINNISPGN TISRSSPKFI GHSPQPLGYD RSSRSSYVDE DCDSLALCDP
421LQRTNCEVDS LPESCFDSGL STLRDPNEYD SEVEYKHQRG FQRSHGVQES FGGDASDTDV
481PDIRDEETFG LEDVASVLDW KPQGSVSEGS IVSSSRKPIS ALSPQTDLVD DNAKSFSSQK
541AYKIVLAGDA AVGKSSFLMR LCKNEFRENI SATLGVDFQM KTLIVDGERT VLQLWDTAGQ
601ERFRSIAKSY FRKADGVLLL YDVTCEKSFL NIREWVDMIE DAAHETVPIM LVGNKADIRD
661TAATEGQKCV PGHFGEKLAM TYGALFCETS AKDGSNIVEA VLHLAREVKK RTDKDDSRSI
721TNLTGTNSKK SPQMKNCCNG
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

740 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.