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RASAL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RASAL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110164-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASAL3 (NM_022904), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAS protein activator like-3 | RAS protein activator like 3 | RASAL3
Origin Species
Human
NCBI GenBank
NM_022904
SwissProt
Q86YV0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPPSPSRTS QTQPTATSPL TSYRWHTGGG GEKAAGGFRW GRFAGWGRAL SHQEPMVSTQ
61PAPRSIFRRV LSAPPKESRT SRLRLSKALW GRHKNPPPEP DPEPEQEAPE LEPEPELEPP
121TPQIPEAPTP NVPVWDIGGF TLLDGKLVLL GGEEEGPRRP RVGSASSEGS IHVAMGNFRD
181PDRMPGKTEP ETAGPNQVHN VRGLLKRLKE KKKARLEPRD GPPSALGSRE SLATLSELDL
241GAERDVRIWP LHPSLLGEPH CFQVTWTGGS RCFSCRSAAE RDRWIEDLRR QFQPTQDNVE
301REETWLSVWV HEAKGLPRAA AGAPGVRAEL WLDGALLART APRAGPGQLF WAERFHFEAL
361PPARRLSLRL RGLGPGSAVL GRVALALEEL DAPRAPAAGL ERWFPLLGAP AGAALRARIR
421ARRLRVLPSE RYKELAEFLT FHYARLCGAL EPALPAQAKE ELAAAMVRVL RATGRAQALV
481TDLGTAELAR CGGREALLFR ENTLATKAID EYMKLVAQDY LQETLGQVVR RLCASTEDCE
541VDPSKCPASE LPEHQARLRN SCEEVFETII HSYDWFPAEL GIVFSSWREA CKERGSEVLG
601PRLVCASLFL RLLCPAILAP SLFGLAPDHP APGPARTLTL IAKVIQNLAN RAPFGEKEAY
661MGFMNSFLEE HGPAMQCFLD QVAMVDVDAA PSGYQGSGDL ALQLAVLHAQ LCTIFAELDQ
721TTRDTLEPLP TILRAIEEGQ PVLVSVPMRL PLPPAQVHSS LSAGEKPGFL APRDLPKHTP
781LISKSQSLRS VRRSESWARP RPDEERPLRR PRPVQRTQSV PVRRPARRRQ SAGPWPRPKG
841SLSMGPAPRA RPWTRDSASL PRKPSVPWQR QMDQPQDRNQ ALGTHRPVNK LAELQCEVAA
901LREEQKVLSR LVESLSTQIR ALTEQQEQLR GQLQDLDSRL RAGSSEFDSE HNLTSNEGHS
961LKNLEHRLNE MERTQAQLRD AVQSLQLSPR TRGSWSQPQP LKAPCLNGDT T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1011 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.