Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RASAL1 / RASAL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130607-100
Catalog Number: LS-G130607
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RASAL1 (NM_001193520), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GAP1 like protein | RASAL1 | RASAL
- Origin Species
- Human
- NCBI GenBank
- NM_004658
- SwissProt
- O95294
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKSSSLNVR VVEGRALPAK DVSGSSDPYC LVKVDDEVVA RTATVWRSLG PFWGEEYTVH61LPLDFHQLAF YVLDEDTVGH DDIIGKISLS REAITADPRG IDSWINLSRV DPDAEVQGEI121CLSVQMLEDG QGRCLRCHVL QARDLAPRDI SGTSDPFARV FWGSQSLETS TIKKTRFPHW181DEVLELREMP GAPSPLRVEL WDWDMVGKND FLGMVEFSPK TLQQKPPKGW FRLLPFPRAE241EDSGGNLGAL RVKVRLIEDR VLPSQCYQPL MELLMESVQG PAEEDTASPL ALLEELTLGD301CRQDLATKLV KLFLGRGLAG RFLDYLTRRE VARTMDPNTL FRSNSLASKS MEQFMKLVGM361PYLHEVLKPV ISRVFEEKKY MELDPCKMDL GRTRRISFKG ALSEEQMRET SLGLLTGYLG421PIVDAIVGSV GRCPPAMRLA FKQLHRRVEE RFPQAEHQQD VKYLAISGFL FLRFFAPAIL481TPKLFDLRDQ HADPQTSRSL LLLAKAVQSI GNLGQQLGQG KELWMAPLHP FLLQCVSRVR541DFLDRLVDVD GDEEAGVPAR ALFPPSAIVR EGYLLKRKEE PAGLATRFAF KKRYVWLSGE601TLSFSKSPEW QMCHSIPVSH IRAVERVDEG AFQLPHVMQV VTQDGTGALH TTYLQCKNVN661ELNQWLSALR KASAPNPNKL AACHPGAFRS ARWTCCLQAE RSAAGCSRTH SAVTLGDWSD721PLDPDAEAQT VYRQLLLGRD QLRLKLLEDS NMDTTLEADT GACPEVLARQ RAATARLLEV781LADLDRAHEE FQQQERGKAA LGPLGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
806 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.