Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RARG / RAR-Gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119410-100
Catalog Number: LS-G119410
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Retinoic Acid Receptor gamma(RARG) (NM_001042728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NR1B3 | RAR-gamma | RARG | Retinoic acid receptor gamma | RARC | RARgamma | Retinoic acid receptor | gamma
- Origin Species
- Human
- NCBI GenBank
- NM_000966
- SwissProt
- P13631
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYDCMETFAP GPRRLYGAAG PGAGLLRRAT GGSCFAGLES FAWPQPASLQ SVETQSTSSE61EMVPSSPSPP PPPRVYKPCF VCNDKSSGYH YGVSSCEGCK GFFRRSIQKN MVYTCHRDKN121CIINKVTRNR CQYCRLQKCF EVGMSKEAVR NDRNKKKKEV KEEGSPDSYE LSPQLEELIT181KVSKAHQETF PSLCQLGKYT TNSSADHRVQ LDLGLWDKFS ELATKCIIKI VEFAKRLPGF241TGLSIADQIT LLKAACLDIL MLRICTRYTP EQDTMTFSDG LTLNRTQMHN AGFGPLTDLV301FAFAGQLLPL EMDDTETGLL SAICLICGDR MDLEEPEKVD KLQEPLLEAL RLYARRRRPS361QPYMFPRMLM KITDLRGIST KGAERAITLK MEIPGPMPPL IREMLENPEM FEDDSSQPGP421HPNASSEDEV PGGQGKGGLK SPATRTRPLEQKLISEEDLAANDILDYKDDDDKV
443 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.