Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RARB / RAR Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101394-100
Catalog Number: LS-G101394
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Retinoic Acid Receptor beta(RARB) (NM_016152), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HBV-activated protein | NR1B2 | RAR Beta | RAR-beta | RAR beta4 | RARB | Retinoic acid receptor beta | RAR beta1 | RAR beta2 | RARbeta | Retinoic acid receptor | beta | RAR beta3 | RAR-epsilon | RARb2 | RRB2
- Origin Species
- Human
- NCBI GenBank
- NM_000965
- SwissProt
- P10826
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.34 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFDCMDVLSV SPGQILDFYT ASPSSCMLQE KALKACFSGL TQTEWQHRHT AQSIETQSTS61SEELVPSPPS PLPPPRVYKP CFVCQDKSSG YHYGVSACEG CKGFFRRSIQ KNMIYTCHRD121KNCVINKVTR NRCQYCRLQK CFEVGMSKES VRNDRNKKKK ETSKQECTES YEMTAELDDL181TEKIRKAHQE TFPSLCQLGK YTTNSSADHR VRLDLGLWDK FSELATKCII KIVEFAKRLP241GFTGLTIADQ ITLLKAACLD ILILRICTRY TPEQDTMTFS DGLTLNRTQM HNAGFGPLTD301LVFTFANQLL PLEMDDTETG LLSAICLICG DRQDLEEPTK VDKLQEPLLE ALKIYIRKRR361PSKPHMFPKI LMKITDLRSI SAKGAERVIT LKMEIPGSMP PLIQEMLENS EGHEPLTPSS421SGNTAEHSPS ISPSSVENSG VSQSPLVQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
448 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.