Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RARA / RAR Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121083-100
Catalog Number: LS-G121083
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Retinoic Acid Receptor alpha(RARA) (NM_001024809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NR1B1 | PML-RAR | RAR-alpha | RARalpha | Retinoic acid receptor alpha | Pml-rara | RAR | RAR alpha | RARA | Retinoic acid receptor | alpha
- Origin Species
- Human
- NCBI GenBank
- NM_000964
- SwissProt
- P10276
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MYESVEVGGP TPNPFLVVDF YNQNRACLLP EKGLPAPGPY STPLRTPLWN GSNHSIETQS61SSSEEIVPSP PSPPPLPRIY KPCFVCQDKS SGYHYGVSAC EGCKGFFRRS IQKNMVYTCH121RDKNCIINKV TRNRCQYCRL QKCFEVGMSK ESVRNDRNKK KKEVPKPECS ESYTLTPEVG181ELIEKVRKAH QETFPALCQL GKYTTNNSSE QRVSLDIDLW DKFSELSTKC IIKTVEFAKQ241LPGFTTLTIA DQITLLKAAC LDILILRICT RYTPEQDTMT FSDGLTLNRT QMHNAGFGPL301TDLVFAFANQ LLPLEMDDAE TGLLSAICLI CGDRQDLEQP DRVDMLQEPL LEALKVYVRK361RRPSRPHMFP KMLMKITDLR SISAKGAERV ITLKMEIPGS MPPLIQEMLE NSEGLDTLSG421QPGGGGRDGG GLAPPPGSCS PSLSPSSNRS SPATHSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
457 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.