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RAPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RAPH1 antibodies →
$725.00each
Catalog Number: LS-G103084-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAPH1 (NM_203365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEQLSDEEID HGAEEDSDKE DQDLDKMFGA WLGELDKLTQ SLDSDKPMEP VKRSPLRQET
61NMANFSYRFS IYNLNEALNQ GETVDLDALM ADLCSIEQEL SSIGSGNSKR QITETKATQK
121LPVSRHTLKH GTLKGLSSSS NRIAKPSHAS YSLDDVTAQL EQASLSMDEA AQQSVLEDTK
181PLVTNQHRRT ASAGTVSDAE VHSISNSSHS SITSAASSMD SLDIDKVTRP QELDLTHQGQ
241PITEHAISLR CSSKQAKRHI DFTEEQAELT PHSYLDRETS LLLRNIAGKP SHLLTKEEQA
301AKLKAEKIRV ALEKIKEAQV KKLVIRVHMS DDSSKTMMVD ERQTVRQVLD NLMDKSHCGY
361SLDWSLVETV SELQMERIFE DHENLVENLL NWTRDSQNKL IFMERIEKYA LFKNPQNYLL
421GKKETAEMAD RNKEVLLEEC FCGSSVTVPE IEGVLWLKDD GKKSWKKRYF LLRASGIYYV
481PKGKAKVSRD LVCFLQLDHV NVYYGQDYRN KYKAPTDYCL VLKHPQIQKK SQYIKYLCCD
541DVRTLHQWVN GIRIAKYGKQ LYMNYQEALK RTESAYDWTS LSSSSIKSGS SSSSIPESQS
601NHSNQSDSGV SDTQPAGHVR SQSIVSSVFS EAWKRGTQLE ESSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

644 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.