Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAPGEF3 / EPAC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100725-100
Catalog Number: LS-G100725
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Epac1(RAPGEF3) (NM_006105), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bcm910 | CAMP-GEFI | CGEF1 | HSU79275 | RAPGEF3 | EPAC | EPAC 1 | EPAC1
- Origin Species
- Human
- NCBI GenBank
- NM_006105
- SwissProt
- O95398
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVLRRMHRPR SCSYQLLLEH QRPSCIQGLR WTPLTNSEES LDFSESLEQA STERVLRAGR61QLHRHLLATC PNLIRDRKYH LRLYRQCCSG RELVDGILAL GLGVHSRSQV VGICQVLLDE121GALCHVKHDW AFQDRDAQFY RFPGPEPEPV GTHEMEEELA EAVALLSQRG PDALLTVALR181KPPGQRTDEE LDLIFEELLH IKAVAHLSNS VKRELAAVLL FEPHSKAGTV LFSQGDKGTS241WYIIWKGSVN VVTHGKGLVT TLHEGDDFGQ LALVNDAPRA ATIILREDNC HFLRVDKQDF301NRIIKDVEAK TMRLEEHGKV VLVLERASQG AGPSRPPTPG RNRYTVMSGT PEKILELLLE361AMGPDSSAHD PTETFLSDFL LTHRVFMPSA QLCAALLHHF HVEPAGGSEQ ERSTYVCNKR421QQILRLVSQW VALYGSMLHT DPVATSFLQK LSDLVGRDTR LSNLLREQWP ERRRCHRLEN481GCGNASPQMK ARNLPVWLPN QDEPLPGSSC AIQVGDKVPY DICRPDHSVL TLQLPVTASV541REVMAALAQE DGWTKGQVLV KVNSAGDAIG LQPDARGVAT SLGLNERLFV VNPQEVHELI601PHPDQLGPTV GSAEGLDLVS AKDLAGQLTD HDWSLFNSIH QVELIHYVLG PQHLRDVTTA661NLERFMRRFN ELQYWVATEL CLCPVPGPRA QLLRKFIKLA AHLKEQKNLN SFFAVMFGLS721NSAISRLAHT WERLPHKVRK LYSALERLLD PSWNHRVYRL ALAKLSPPVI PFMPLLLKDM781TFIHEGNHTL VENLINFEKM RMMARAARML HHCRSHNPVP LSPLRSRVSH LHEDSQVARI841STCSEQSLST RSPASTWAYV QQLKVIDNQR ELSRLSRELE PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
881 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.