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RANBP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RANBP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RANBP6 antibodies →
$504.00each
Catalog Number: LS-G114440-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RANBP6 (NM_012416), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAN binding protein 6 | Ran-binding protein 6 | RANBP6 | Ran-GTP binding protein
Origin Species
Human
NCBI GenBank
AF039023
SwissProt
O60518

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAATASAGVP ATVSEKQEFY QLLKNLINPS CMVRRQAEEI YENIPGLCKT TFLLDAVRNR
61RAGYEVRQMA AALLRRLLSS GFEEVYPNLP ADVQRDVKIE LILAVKLETH ASMRKKLCDI
121FAVLARNLID EDGTNHWPEG LKFLIDSIYS KNVVLWEVAL HVFWHFPGIF GTQERHDLDI
181IKRLLDQCIQ DQEHPAIRTL SARAAAAFVL ANENNIALFK DFADLLPGIL QAVNDSCYQD
241DDSVLESLVE IADTVPKYLG PYLEDTLQLS LKLCGDSRLS NLQRQLALEV IVTLSETATP
301MLKKHTNIIA QAVPHILAMM VDLQDDEDWV NADEMEEDDF DSNAVAAESA LDRLACGLGG
361KVVLPMTKEH IMQMLQSPDW KYRHAGLMAL SAIGEGCHQQ MESILDETVN SVLLFLQDPH
421PRVRAAACTT LGQMATDFAP NFQKKFHETV IAALLRTMEN QGNQRVQSHA ASALIIFIED
481CPKSLLVLYV DSMVKNLHSV LVIKLQELIR NGTKLALEQL VTTIASVADT IEEKFVPYYD
541IFMPSLKHIV ELAVQKELKL LRGKTIECIS HIGLAVGKEK FMQDASNVMQ LLLKTQSDLN
601NMEDDDPQTS YMVSAWARMC KILGKDFQQY LPLVIEPLIK TASAKPDVAL LDTQDVENMS
661DDDGWQFVNL GDQQSFGIKT SGLEAKATAC QMLVYYAKEL REGFVEYTEQ VVKLMVPLLK
721FYFHDNVRVA AAESMPFLLE CARIRGPEYL AQMWQFICDP LIKAIGTEPD TDVLSEIMNS
781FAKSIEVMGD GCLNDEHLEE LGGILKAKLE GHFKNQELRQ VKRQEENYDQ QVEMSLQDED
841ECDVYILTKV SDILHSLFST YKEKILPWFE QLLPLIVNLI CSSRPWPDRQ WGLCIFDDII
901EHCSPTSFKY VEYFRWPMLL NMRDNNPEVR QAAAYGLGVM AQFGGDDYRS LCSEAVPLLV
961KVIKCANSKT KKNVIATENC ISAIGKILKF KPNCVNVDEV LPHWLSWLPL HEDKEEAIQT
1021LSFLCDLIES NHPVVIGPNN SNLPKIISII AEGKINETIN YEDPCAKRLA NVVRQVQTSE
1081DLWLECVSQL DDEQQEALQE LLNFA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1105 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.