LSBio
RANBP3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RANBP3L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RANBP3L antibodies →
$504.00each
Catalog Number: LS-G128183-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RANBP3L (NM_001161429), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ran-binding protein 3-like | RAN binding protein 3-like | RANBP3L
Origin Species
Human
NCBI GenBank
NM_001161429
SwissProt
Q86VV4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTIPRKGSS HLPGSLHTCK LKLQEDRRQQ EKSVIAQPIF VFEKGEQTFK RPAEDTLYEA
61AEPECNGFPT KRVRSSSFTF HITDSQSQGV STLSQKQMRC SSVTNLPTFP HSGPVRKNNV
121FMTSALVQSS VDIKSAEQGP VKHSKHVIRP AILQLPQARS CAKVRKTFGH KALESCKTKE
181KTNNKISEGN SYLLSENLSR ARISVQLSTN QDFLGATSVG CQPNEDKCSF KSCSSNFVFG
241ENMVERVLGT QKLTQPQLEN DSYAKEKPFK SIPKFPVNFL SSRTDSIKNT SLIESAAAFS
301SQPSRKCLLE KIDVITGEET EHNVLKINCK LFIFNKTTQS WIERGRGTLR LNDTASTDCG
361TLQSRLIMRN QGSLRLILNS KLWAQMKIQR ANHKNVRITA TDLEDYSIKI FLIQASAQDT
421AYLYAAIHHR LVALQSFNKQ RDVNQAESLS ETAQQLNCES CDENEDDFIQ VTKNGSDPSS
481WTHRQSVACS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.