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RANBP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RANBP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RANBP10 antibodies →
$504.00each
Catalog Number: LS-G110954-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RANBP10 (NM_020850), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1464 | RANBP10 | Ran-binding protein 10 | RAN binding protein 10
Origin Species
Human
NCBI GenBank
AB040897
SwissProt
Q6VN20

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAATADPGA GNPQPGDSSG GGAGGGLPSP GEQELSRRLQ RLYPAVNQQE TPLPRSWSPK
61DKYNYIGLSQ GNLRVHYKGH GKNHKDAASV RATHPIPAAC GIYYFEVKIV SKGRDGYMGI
121GLSAQGVNMN RLPGWDKHSY GYHGDDGHSF CSSGTGQPYG PTFTTGDVIG CCVNLINGTC
181FYTKNGHSLG IAFTDLPANL YPTVGLQTPG EIVDANFGQQ PFPFDIEDYM REWRAKVQGT
241VHCFPISARL GEWQAVLQNM VSSYLVHHGY CATATAFARM TETPIQEEQA SIKNRQKIQK
301LVLEGRVGEA IETTQRFYPG LLEHNPNLLF MLKCRQFVEM VNGTDSEVRS LSSRSPKSQD
361SYPGSPSLSP RHGPSSSHMH NTGADSPSCS NGVASTKSKQ NHSKYPAPSS SSSSSSSSSS
421SSPSSVNYSE SNSTDSTKSQ HHSSTSNQET SDSEVEMEAE HYPNGVLGSM STRIVNGAYK
481HEDLQTDESS MDDRHPRRQL CGGNQAATER IILFGRELQA LSEQLGREYG KNLAHTEMLQ
541DAFSLLAYSD PWSCPVGQQL DPIQREPVCA ALNSAILESQ NLPKQPPLML ALGQASECLR
601LMARAGLGSC SFARVDDYLH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.