Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAIG2 / GPRC5B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101408-100
Catalog Number: LS-G101408
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPRC5B (NM_016235), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A-69G12.1 | GPRC5B | RAIG2 | RAIG-2
- Origin Species
- Human
- NCBI GenBank
- NM_016235
- SwissProt
- Q9NZH0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.79 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD LDAIWGIVVE61AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL LGTLGLFGLT FAFIIQEDET121ICSVRRFLWG VLFALCFSCL LSQAWRVRRL VRHGTGPAGW QLVGLALCLM LVQVIIAVEW181LVLTVLRDTR PACAYEPMDF VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA241FLSVLIWVAW MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL301QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF PNGSLGKRPS361GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR HLWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.