LSBio
RAI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RAI2 antibodies →
$504.00each
Catalog Number: LS-G129972-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAI2 (NM_001172743), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAI2 | Retinoic acid induced 2
Origin Species
Human
NCBI GenBank
NM_001172743

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDDLQSQNLS MDMTDSPPAL ANNRLENGMA QLITTEAWNI NSTDLVKKAL VTVPAPSILN
61PPAESQSGMA LKVAATVLQP LCLGESPVVM PIHMQVEGSS APELNPNGNA TYVMTTQGPV
121QLPVVLEQHV FQHLNSPLVL PQEAPCSSST IHNNLFQGAE DPEAQPQLLD LRIPSQPQEP
181TLPFEAVLQN LFPSQGTLGP PPCQPPPGYA PVPPQPFSSP LSPLVPPATL LVPYPVIVPL
241PVPVPIPIPI PVPQSSESKF SSSFPKPPSS FGLHPFKGTQ TPLEKDELKP FDILQPKEYF
301QLSRHTVIKM GSENEALDLS MKSVPWLKAG EVSPPIFQED APLDLSVAAH RKSEPPPETL
361YDSGASVDSS GHTVMEKLPS GMEISFAPAT SHEAPAMMDS HISSSDAATE MLSQPNHPSG
421EVKAENNIEM VGESQAAKVI VSVEDAVPTI FCGKIKGLSG VSTKNFSFKR EDSVLQGYDI
481NSQGEESMGN AEPLRKPIKN RSIKLKKVNS QEIHMLPIKK QRLATFFPRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.