Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAG2 / RAG-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G123130-100
Catalog Number: LS-G123130
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAG2 (NM_000536), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RAG-2 | RAG2
- Origin Species
- Human
- NCBI GenBank
- M94633
- SwissProt
- P55895
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLQMVTVSN NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHLDV KHNHVKLKPT61IFSKDSCYLP PLRYPATCTF KGSLESEKHQ YIIHGGKTPN NEVSDKIYVM SIVCKNNKKV121TFRCTEKDLV GDVPEARYGH SINVVYSRGK SMGALFGGRS YMPSTHRTTE KWNSVADCLP181CVFLVDFEFG CATSYILPEL QDGLSFHVSI AKNDTIYILG GHSLANNIRP ANLYRIRVDL241PLGSPAVNCT VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMICNII SLEDNKIEIR301EMETPDWTPD IKHSKIWFGS NTGNGTVFLG IPGDNKQVVS EGFYFYMLKC AEDDTNEEQT361TFTNSQTSTE DPGDSTPFED SEEFCFSAEA NSFDGDDEFD TYNEDDEEDE SETGYWITCC421PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LAERTLIHLS AGSNKYYCNE481HVEIARALHT PQRVLPLKKP PMKSLRKKGS GKILTPAKKS FLRRLFDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.