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RADIL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RADIL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RADIL antibodies →
$725.00each
Catalog Number: LS-G112022-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RADIL (NM_018059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_018059
SwissProt
Q96JH8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFYGTHFIMS PPTKSKLKRQ SQLLSSMLSR TLSYKYRDLD STFSSLGASD DPAELSTQLS
61APGVLKVFGD SVCTGTHYKS VLATGTSSAR ELVKEALERY ALDPRQAGQY VLCDVVGQAG
121DAGQRWQARC FRVFGDSEKP LLIQELWKPR EGLSRRFELR KRSDVEELAA KEVDTITAGI
181NAQARRLQRS RAKGTPTPAL GDARSSPPPR LRRTVSETSL SPVNALPAAA QGPEEPGPDA
241MRYSLYQSPH LLLLQGYSQQ HDSLVYVLNR DRHTVGQRTP SSKPSISLSA PDILPLHCTI
301RRQPLPDSGQ AAGRLVLEPI PGAHISVNFS EVGHRTVVLH HGDLLSLGLY YLLLFKDPAQ
361AQPLPARALA RLRAVPQSCR LCGAALGARG AASPTQAALP RRQQLLLEFE PHLEDTLLQR
421IMTLIEPGGD DHKLTPAFLL CLCIQHSATH FQPGTFGQLL LKIARLIRET VWEKTKELAE
481KQAQLQEPIS LASCAMADLV PDLQPILFWM SNSIELLYFI QQKCPLYMQS MEEQLDITGS
541KESLFSCTLT ASEEAMAVLE EVVLYAFQQC VYYVSKSLYI CLPALLECPP FQTERRESWS
601SAPELPEELR RVVSVYQAAL DLLRQLQVHP EVASQMLAYL FFFSGTLLLN QLLDRGPSLS
661CFHWPRGVQA CARLQQLLEW MRSAGFGAAG EHFFQKLSCT LNLLATPRAQ LIQMSWTALR
721AAFPALSPAQ LHRLLTHYQL ASAMGPMSTW EPGAQDSPEA FRSEDVLESY ENPPPIVLPS
781DGFQVDLEAN CLDDSIYQHL LYVRHFLWGL RSRASPGSPG RPGSGASQPV CPEGMHHVVL
841DGHLEAPSCP LAPRDPGPAA REVAPERTLP LRGAPWAQAP PGRQPGRGGS QAGPPHTDSS
901CLLTPPSTPL GPEPGDPDWP ESGGPCGKAL PERQRNGLSG LRGAAPEGDS AALAEESPPA
961PSSRSSSTED FCYVFTVELE RGPSGLGMGL IDGMHTHLGA PGLYIQTLLP GSPAAADGRL
1021SLGDRILEVN GSSLLGLGYL RAVDLIRHGG KKMRFLVAKS DVETAKKIHF RTPPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1075 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.