Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAD51 / RECA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128056-100
Catalog Number: LS-G128056
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAD51 (NM_001164269), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRCC5 | HsT16930 | RAD51 | RAD51 homolog A | RAD51 homolog (S. cerevisiae) | RecA | E. coli | homolog of | RecA-like protein | Recombination protein A | MRMV2 | HRAD51 | HsRad51 | RAD51A | RECA
- Origin Species
- Human
- NCBI GenBank
- NM_002875
- SwissProt
- Q06609
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL61INIKGISEAK ADKILTESRS VARLECNSVI LVYCTLRLSG SSDSPASASR VVGTTGGIET121GSITEMFGEF RTGKTQICHT LAVTCQLPID RGGGEGKAMY IDTEGTFRPE RLLAVAERYG181LSGSDVLDNV AYARAFNTDH QTQLLYQASA MMVESRYALL IVDSATALYR TDYSGRGELS241ARQMHLARFL RMLLRLADEF GVAVVITNQV VAQVDGAAMF AADPKKPIGG NIIAHASTTR301LYLRKGRGET RICKIYDSPC LPEAEAMFAI NADGVGDAKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
340 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.