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RAD17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAD17 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RAD17 antibodies →
$504.00each
Catalog Number: LS-G117708-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAD17 (NM_002873), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HRAD17 | R24L | RAD1 homolog | RAD1 (S. pombe) homolog | RAD17 | RAD24 | Rad17-like protein | RF-C activator 1 homolog | CCYC | RAD17 homolog (S. pombe) | RAD17SP | RF-C/activator 1 homolog
Origin Species
Human
NCBI GenBank
NM_002873
SwissProt
O75943

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNQVTDWVDP SFDDFLECSG VSTITATSLG VNNSSHRRKN GPSTLESSRF PARKRGNLSS
61LEQIYGLENS KEYLSENEPW VDKYKPETQH ELAVHKKKIE EVETWLKAQV LERQPKQGGS
121ILLITGPPGC GKTTTLKILS KEHGIQVQEW INPVLPDFQK DDFKGMFNTE SSFHMFPYQS
181QIAVFKEFLL RATKYNKLQM LGDDLRTDKK IILVEDLPNQ FYRDSHTLHE VLRKYVRIGR
241CPLIFIISDS LSGDNNQRLL FPKEIQEECS ISNISFNPVA PTIMMKFLNR IVTIEANKNG
301GKITVPDKTS LELLCQGCSG DIRSAINSLQ FSSSKGENNL RPRKKGMSLK SDAVLSKSKR
361RKKPDRVFEN QEVQAIGGKD VSLFLFRALG KILYCKRASL TELDSPRLPS HLSEYERDTL
421LVEPEEVVEM SHMPGDLFNL YLHQNYIDFF MEIDDIVRAS EFLSFADILS GDWNTRSLLR
481EYSTSIATRG VMHSNKARGY AHCQGGGSSF RPLHKPQWFL INKKYRENCL AAKALFPDFC
541LPALCRQTQL LPYLALLTIP MRNQAQISFI QDIGRLPLKR HFGRLKMEAL TDREHGMIDP
601DSGDEAQLNG GHSAEESLGE PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI
661IIEDYESDGT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.