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RABGAP1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RABGAP1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113654-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RABGAP1L (NM_014857), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HHL | TBC1 domain family | member 18 | RP1-102G20.1 | KIAA0471 | RABGAP1L | TBC1D18
Origin Species
Human
NCBI GenBank
NM_014857
SwissProt
Q5R372

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVRASLQKV SGSSDSVATM NSEEFVLVPQ YADDNSTKHE EKPQLKIVSN GDEQLEKAME
61EILRDSEKRP SSLLVDCQSS SEISDHSFGD IPASQTNKPS LQLILDPSNT EISTPRPSSP
121GGLPEEDSVL FNKLTYLGCM KVSSPRNEVE ALRAMATMKS SSQYPFPVTL YVPNVPEGSV
181RIIDQSSNVE IASFPIYKVL FCARGHDGTT ESNCFAFTES SHGSEEFQIH VFSCEIKEAV
241SRILYSFCTA FKRSSRQVSD VKDSVIPTPD SDVFTFSVSL EVKEDDGKGN FSPVPKDRDK
301FYFKLKQGIE KKVVITVQQL SNKELAIERC FGMLLSPGRN VKNSDMHLLD MESMGKSYDG
361RAYVITGMWN PNAPVFLALN EETPKDKQVY MTVAVDMVVT EVVEPVRFLL ETVVRVYPAN
421ERFWYFSRKT FTETFFMRLK QSEGKGHTNA GDAIYEVVSL QRESDKEEPV TPTSGGGPMS
481PQDDEAEEES DNELSSGTGD VSKDCPEKIL YSWGELLGKW HSNLGARPKG LSTLVKSGVP
541EALRAEVWQL LAGCHDNQAM LDRYRILITK DSAQESVITR DIHRTFPAHD YFKDTGGDGQ
601ESLYKICKAY SVYDEDIGYC QGQSFLAAVL LLHMPEEQAF CVLVKIMYDY GLRDLYRNNF
661EDLHCKFYQL ERLMQEQLPD LHSHFSDLNL EAHMYASQWF LTLFTAKFPL CMVFHIIDLL
721LCEGLNIIFH VALALLKTSK EDLLQADFEG ALKFFRVQLP KRYRAEENAR RLMEQACNIK
781VPTKKLKKYE KEYQTMRESQ LQQEDPMDRY KFVYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

815 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.