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RABEP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RABEP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109727-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RABEP2 (NM_024816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_024816
SwissProt
Q9H5N1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAPVAAD DDERRRRPGA ALEDSRSQEG ANGEAESGEL SRLRAELAGA LAEMETMKAV
61AEVSESTKAE AVAAVQRQCQ EEVASLQAIL KDSISSYEAQ ITALKQERQQ QQQDCEEKER
121ELGRLKQLLS RAYPLDSLEK QMEKAHEDSE KLREIVLPME KEIEELKAKL LRAEELIQEI
181QRRPRHAPSL HGSTELLPLS RDPSPPLEPL EELSGDGGPA AEAFAHNCDD SASISSFSLG
241GGVGSSSSLP QSRQGLSPEQ EETASLVSTG TLVPEGIYLP PPGYQLVPDT QWEQLQTEGR
301QLQKDLESVS RERDELQEGL RRSNEDCAKQ MQVLLAQVQN SEQLLRTLQG TVSQAQERVQ
361LQMAELVTTH KCLHHEVKRL NEENQGLRAE QLPSSAPQGS QQEQGEEESL PSSVPELQQL
421LCCTRQEARA RLQAQEHGAE RLRIEIVTLR EALEEETVAR ASLEGQLRVQ REETEVLEAS
481LCSLRTEMER VQQEQSKAQL PDLLSEQRAK VLRLQAELET SEQVQRDFVR LSQALQVRLE
541RIRQAETLEQ VRSIMDEAPL TDVRDIKDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.