Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAB3GAP2 / p150 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114438-100
Catalog Number: LS-G114438
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAB3GAP2 (NM_012414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DKFZP434D245 | p150 | KIAA0839 | Rab3-GAP p150 | RAB3-GAP150 | RAB3GAP2 | RAB3GAP150 | RGAP-iso | WARBM2 | RP11-568G11.1 | Rab3-GAP regulatory subunit
- Origin Species
- Human
- NCBI GenBank
- NM_012414
- SwissProt
- Q9H2M9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 155.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MACSIVQFCY FQDLQAARDF LFPHLREEIL SGALRRDPSK STDWEDDGWG AWEENEPQEP61EEEGNTCKTQ KTSWLQDCVL SLSPTNDLMV IAREQKAVFL VPKWKYSDKG KEEMQFAVGW121SGSLNVEEGE CVTSALCIPL ASQKRSSTGR PDWTCIVVGF TSGYVRFYTE NGVLLLAQLL181NEDPVLQLKC RTYEIPRHPG VTEQNEELSI LYPAAIVTID GFSLFQSLRA CRNQVAKAAA241SGNENIQPPP LAYKKWGLQD IDTIIDHASV GIMTLSPFDQ MKTASNIGGF NAAIKNSPPA301MSQYITVGSN PFTGFFYALE GSTQPLLSHV ALAVASKLTS ALFNAASGWL GWKSKHEEEA361VQKQKPKVEP ATPLAVRFGL PDSRRHGESI CLSPCNTLAA VTDDFGRVIL LDVARGIAIR421MWKGYRDAQI GWIQTVEDLH ERVPEKADFS PFGNSQGPSR VAQFLVIYAP RRGILEVWST481QQGPRVGAFN VGKHCRLLYP GYKIMGLNNV TSQSWQPQTY QICLVDPVSG SVKTVNVPFH541LALSDKKSER AKDMHLVKKL AALLKTKSPN LDLVETEIKE LILDIKYPAT KKQALESILA601SERLPFSCLR NITQTLMDTL KSQELESVDE GLLQFCANKL KLLQLYESVS QLNSLDFHLD661TPFSDNDLAL LLRLDEKELL KLQALLEKYK QENTRTNVRF SDDKDGVLPV KTFLEYLEYE721KDVLNIKKIS EEEYVALGSF FFWKCLHGES STEDMCHTLE SAGLSPQLLL SLLLSVWLSK781EKDILDKPQS ICCLHTMLSL LSKMKVAIDE TWDSQSVSPW WQQMRTACIQ SENNGAALLS841AHVGHSVAAQ ISNNMTEKKF SQTVLGADSE ALTDSWEALS LDTEYWKLLL KQLEDCLILQ901TLLHSKGNTQ TSKVSSLQAE PLPRLSVKKL LEGGKGGIAD SVAKWIFKQD FSPEVLKLAN961EERDAENPDE PKEGVNRSFL EVSEMEMDLG AIPDLLHLAY EQFPCSLELD VLHAHCCWEY1021VVQWNKDPEE ARFFVRSIEH LKQIFNAHVQ NGIALMMWNT FLVKRFSAAT YLMDKVGKSP1081KDRLCRRDVG MSDTAMTSFL GSCLDLLQIL MEADVSRDEI QVPVLDTEDA WLSVEGPISI1141VELALEQKHI HYPLVEHHSI LCSILYAVMR FSLKTVKPLS LFDSKGKNAF FKDLTSIQLL1201PSGEMDPNFI SVRQQFLLKV VSAAVQAQHS ATKVKDPTEE ATPTPFGKDQ DWPALAVDLA1261HHLQVSEDVV RRHYVGELYN YGVDHLGEEA ILQVHDKEVL ASQLLVLTGQ RLAHALLHTQ1321TKEGMELLAR LPPTLCTWLK AMDPQDLQNT EVPIATTAKL VNKVIELLPE KHGQYGLALH1381LIEAVEAISL PSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1393 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.