Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAB11FIP5 / RIP11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113192-100
Catalog Number: LS-G113192
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAB11FIP5 (NM_015470), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gaf-1 | KIAA0857 | GAF1 | Phosphoprotein pp75 | Rab11-FIP5 | RAB11RIP5 | RAB11FIP5 | Gamma-SNAP-associated factor 1 | Pp75 | RIP11
- Origin Species
- Human
- NCBI GenBank
- NM_015470
- SwissProt
- Q9BXF6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV61EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA SSAAACELVL TTMHRSLIGV121DKFLGQATVA LDEVFGAGRA QHTQWYKLHS KPGKKEKERG EIEVTIQFTR NNLSAMFDLS181MKDKPRSPFS KIRDKMKGKK KYDLESASAI LPSSAIEDPD LGSLGKMGKA KGFFLRNKLR241KSSLTQSNTS LGSDSTLSSA SGSLAYQGPG AELLTRSPSR SSWLSTEGGR DSAQSPKLFT301HKRTYSDEAN QMRVAPPRAL LDLQGHLDAA SRSSLCVNGS HIYNEEPQGP VRHRSSISGS361LPSSGSLQAV SSRFSEEGPR STDDTWPRGS RSNSSSEAVL GQEELSAQAK VLAPGASHPG421EEEGARLPEG KPVQVATPIV ASSEAVAEKE GARKEERKPR MGLFHHHHQG LSRSELGRRS481SLGEKGGPIL GASPHHSSSG EGKAKSSWFG LREAKDPTQK PSPHPVKPLS AAPVEGSPDR541KQSRSSLSIA LSSGLEKLKT VTSGSIQPVT QAPQAGQMVD TKRLKDSAVL DQSAKYYNLT601HDELISLLLQ RERELSQRDE HVQELESYID RLLVRIMETS PTLLQIPPGP PKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
652 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.