Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RAB11FIP2 / Rab11-FIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113566-100
Catalog Number: LS-G113566
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAB11 FIP2(RAB11FIP2) (NM_014904), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0941 | NRip11 | Rab11-FIP2 | RAB11-FIP2 long isoform | RAB11FIP2
- Origin Species
- Human
- NCBI GenBank
- NM_014904
- SwissProt
- Q7L804
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMLSEQAQKW FPTHVQVTVL QAKDLKPKGK SGTNDTYTII QLGKEKYSTS VAEKTLEPVW61KEEASFELPG LLIQGSPEKY ILFLIVMHRS LVGLDKFLGQ VAINLNDIFE DKQRRKTEWF121RLESKQGKRI KNRGEIKVNI QFMRNNMTAS MFDLSMKDKT RSPFAKLKDK MKGRKNDGTF181SDTSSAIIPS THMPDANSEF SSGEIQMKSK PKKPFLLGPQ RLSSAHSMSD LSGSHMSSEK241LKAGTIGQTH LLGHQLDSFG TVPESGSLKS PHRRTLSFDT SKMNQPDSIV DEGELCFGRQ301NDPFTNVTAS LPQKFATLPR KKNPFEESSE TWDSSMNLFS KPIEIRKENK REKREKVSLF361ERVTGKKDSR RSDKLNNGGS DSPCDLKSPN AFSENRQDYF DYESTNPFTA KFRASNIMPS421SSFHMSPTSN EDLRKIPDSN PFDATAGYRS LTYEEVLQEL VKHKELLRRK DTHIRELEDY481IDNLLVRVME ETPSILRVPY EPSRKAGKFS NSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
512 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.