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RAB11FIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RAB11FIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RAB11FIP1 antibodies →
$504.00each
Catalog Number: LS-G109559-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAB11FIP1 (NM_025151), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Rab11-FIP1 | Rab effector protein | Rab-coupling protein | NOEL1A | RAB11 coupling protein | RAB11FIP1 | RCP
Origin Species
Human
NCBI GenBank
NM_025151

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLMVSAGRG LGAVWSPTHV QVTVLQARGL RAKGPGGTSD AYAVIQVGKE KYATSVSERS
61LGAPVWREEA TFELPSLLSS GPAAAATLQL TVLHRALLGL DKFLGRAEVD LRDLHRDQGR
121RKTQWYKLKS KPGKKDKERG EIEVDIQFMR NNMTASMFDL SMKDKSRNPF GKLKDKIKGK
181NKDSGSDTAS AIIPSTTPSV DSDDESVVKD KKKKSKIKTL LSKSNLQKTP LSQSMSVLPT
241SKPEKVLLRP GDFQSQWDED DNEDESSSAS DVMSHKRTAS TDLKQLNQVN FTLPKKEGLS
301FLGGLRSKND VLSRSNVCIN GNHVYLEQPE AKGEIKDSSP SSSPSPKGFR KKHLFSSTEN
361LAAGSWKEPA EGGGLSSDRQ LSESSTKDSL KSMTLPSYRP APLVSGDLRE NMAPANSEAT
421KEAKESKKPE SRRSSLLSLM TGKKDVAKGS EGENPLTVPG REKEGMLMGV KPGEDASGPA
481EDLVRRSEKD TAAVVSRQGS SLNLFEDVQI TEPEAEPESK SEPRPPISSP RAPQTRAVKP
541RLHPVKPMNA MATKVANCSL GTATIISENL NNEVMMKKYS PSDPAFAYAQ LTHDELIQLV
601LKQKETISKK EFQVRELEDY IDNLLVRVME ETPNILRIPT QVGKKAGKM
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

649 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.