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R3HDM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

R3HDM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all R3HDM2 antibodies →
$725.00each
Catalog Number: LS-G113598-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human R3HDM2 (NM_014925), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1002 | PR01365 | R3H domain containing 2 | R3HDM2
Origin Species
Human
NCBI GenBank
NM_014925
SwissProt
Q9Y2K5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNSNTTQET LEIMKESEKK LVEESVNKNK FISKTPSKEE IEKECEDTSL RQETQRRTSN
61HGHARKRAKS NSKLKLVRSL AVCEESSTPF ADGPLETQDI IQLHISCPSD KEEEKSTKDV
121SEKEDKDKNK EKIPRKMLSR DSSQEYTDST GIDLHEFLVN TLKKNPRDRM MLLKLEQEIL
181EFINDNNNQF KKFPQMTSYH RMLLHRVAAY FGMDHNVDQT GKAVIINKTS NTRIPEQRFS
241EHIKDEKNTE FQQRFILKRD DASMDRDDNQ TGQNGYLNDI RLSKEAFSSS SHKRRQIFRG
301NREGLSRTSS SRQSSTDSEL KSLEPRPWSS TDSDGSVRSM RPPVTKASSF SGISILTRGD
361SIGSSKGGSA GRISRPGMAL GAPEVCNQVT SSQSVRGLLP CTAQQQQQQQ QQQLPALPPT
421PQQQPPLNNH MISQADDLSN PFGQMSLSRQ GSTEAADPSA ALFQTPLISQ HPQQTSFIMA
481STGQPLPTSN YSTSSHAPPT QQVLPPQGYM QPPQQIQVSY YPPGQYPNSN QQYRPLSHPV
541AYSPQRGQQL PQPSQQPGLQ PMMPNQQQAA YQGMIGVQQP QNQGLLSSQR SSMGGQMQGL
601VVQYTPLPSY QVPVGSDSQN VVQPPFQQPM LVPVSQSVQG GLPAAGVPVY YSMIPPAQQN
661GTSPSVGFLQ PPGSEQYQMP QSPSPCSPPQ MPQQYSGVSP SGPGVVVMQL NVPNGPQPPQ
721NPSMVQWSHC KYYSMDQRGQ KPGDLYSPDS SPQANTQMSS SPVTSPTQSP APSPVTSLSS
781VCTGLSPLPV LTQFPRPGGP AQGDGRYSLL GQPLQYNLSI CPPLLHGQST YTVHQGQSGL
841KHGNRGKRQA LKSASTDLGT ADVVLGRVLE VTDLPEGITR TEADKLFTQL AMSGAKIQWL
901KDAQGLPGGG GGDNSGTAEN GRHSDLAALY TIVAVFPSPL AAQNASLRLN NSVSRFKLRM
961AKKNYDLRIL ERASSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

976 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.