Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
R3HCC1L / C10orf28 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113920-100
Catalog Number: LS-G113920
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GIDRP88(R3HCC1L) (NM_014472), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf28 | GIDRP86 | GIDRP88 | PSORT | R3HCC1L
- Origin Species
- Human
- NCBI GenBank
- AF050198
- SwissProt
- Q7Z5L2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQQESERCRV RARRPDMALY VPKARRGAVL LKTGDEEESC GSPNSVVKEK QKESSLSQKE61VFKDKPEARR LNINPDRKEH NCREEKKSST KLRMDTCLQK TNRVCSKRGT TESKEVLSQG121QQQGAPNAGV ITNAPLQRHF KPKKVECLEV ETTDVTGHER ILLSQACLEI SEAQVPSKPF181QNVEFCDFSR HEPDGEAFED KDLEGRIETD TKVLEILYEF PRVFSSVMKP ENMIVPIKLS241SDSEIVQQSM QTSDGILNPS SGGITTTSVP GSPDGVFDQT CVDFEVESVG GIANSTGFIL301DQKDTDSIPA TMGHISLSES TNDTVSPVMI RECEKNDSTA DELHVKHEPP DTAVLAHETH361RDSGFKNVGD ITNKACMMDT TGMSCSDHVT VDSPYVVAVR IADETSINTR SFSKFVGMSA421DATPLHVARS GNDTEDFSNP SACSDIYGES ISSHFTESTG KLIESLSDCA SSLPIKKIAG481SNYNTFLDSE LSMLNGTKVL SDSAVGIDLG STGDTTEALH ELRTAEEFKT EEQDASGSIE541FGVSFPDRES SSMETSIEPK ATETSHTEGI TAIEESWESM FNDDGDCLDP RLLQELSGNT601KSRESIQEPR SDYYNHEVPD IDLSDCEFPH VIEIYDFPQE FRTEDLLRVF CSYQKKGFDI661KWVDDTHALG VFSSPITARD ALGIKHTMVK IRPLSQATRA AKAKARAYAE FLQPAKERPE721TSAALARRLV ISALGVRSKQ SKTEREAELK KLQEARERKR LEAKQREDIW EGRDQSTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
778 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.