Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
QRSL1 / GatA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111817-100
Catalog Number: LS-G111817
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human QRSL1 (NM_018292), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DKFZP564C1278 | Glu-AdT subunit A | GatA | QRSL1
- Origin Species
- Human
- NCBI GenBank
- NM_018292
- SwissProt
- Q9H0R6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGRSLREVS AALKQGQITP TELCQKCLSL IKKTKFLNAY ITVSEEVALK QAEESEKRYK61NGQSLGDLDG IPIAVKDNFS TSGIETTCAS NMLKGYIPPY NATVVQKLLD QGALLMGKTN121LDEFAMGSGS TDGVFGPVKN PWSYSKQYRE KRKQNPHSEN EDSDWLITGG SSGGSAAAVS181AFTCYAALGS DTGGSTRNPA AHCGLVGFKP SYGLVSRHGL IPLVNSMDVP GILTRCVDDA241AIVLGALAGP DPRDSTTVHE PINKPFMLPS LADVSKLCIG IPKEYLVPEL SSEVQSLWSK301AADLFESEGA KVIEVSLPHT SYSIVCYHVL CTSEVASNMA RFDGLQYGHR CDIDVSTEAM361YAATRREGFN DVVRGRILSG NFFLLKENYE NYFVKAQKVR RLIANDFVNA FNSGVDVLLT421PTTLSEAVPY LEFIKEDNRT RSAQDDIFTQ AVNMAGLPAV SIPVALSNQG LPIGLQFIGR481AFCDQQLLTV AKWFEKQVQF PVIQLQELMD DCSAVLENEK LASVSLKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
528 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.