Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
QRFPR / GPR103 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102564-100
Catalog Number: LS-G102564
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR103(QRFPR) (NM_198179), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AQ27 | G protein-coupled receptor 103 | QRFP receptor | QRFPR | SP9155 | G-protein coupled receptor 103 | GPR103
- Origin Species
- Human
- NCBI GenBank
- NM_198179
- SwissProt
- Q96P65
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQALNITPEQ FSRLLRDHNL TREQFIALYR LRPLVYTPEL PGRAKLALVL TGVLIFALAL61FGNALVFYVV TRSKAMRTVT NIFICSLALS DLLITFFCIP VTMLQNISDN WLGGAFICKM121VPFVQSTAVV TEILTMTCIA VERHQGLVHP FKMKWQYTNR RAFTMLGVVW LVAVIVGSPM181WHVQQLEIKY DFLYEKEHIC CLEEWTSPVH QKIYTTFILV ILFLLPLMVM LILYSKIGYE241LWIKKRVGDG SVLRTIHGKE MSKIARKKKR AVIMMVTVVA LFAVCWAPFH VVHMMIEYSN301FEKEYDDVTI KMIFAIVQII GFSNSICNPI VYAFMNENFK KNVSSAVCYC IVNKTFSPAQ361RHGNSGITMM RKKAKFSLRE NPVEETKGEA FSDGNIEVKL CEQTEEKKKL KRHLALFRSE421LAENSPLDSG HTRTRPLEQKLISEEDLAANDILDYKDDDDKV
431 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.