Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PYGO1 / Pygopus 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113128-100
Catalog Number: LS-G113128
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PYGO1 (NM_015617), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PYGO1 | Pygopus 1 | Pygopus homolog 1 (Drosophila) | Pygopus homolog 1 | Pygopus-like protein 1
- Origin Species
- Human
- SwissProt
- Q9Y3Y4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPAENSPAPA YKVSSHGGDS GLDGLGGPGV QLGSPDKKKR KANTQGPSFP PLSEYAPPPN61PNSDHLVAAN PFDDNYNTIS YKPLPSSNPY LGPGYPGFGG YSTFRMPPHV PPRMSSPYCG121PYSLRNQPHP FPQNPLGMGF NRPHAFNFGP HDNSSFGNPS YNNALSQNVN MPNQHFRQNP181AENFSQIPPQ NASQVSNPDL ASNFVPGNNS NFTSPLESNH SFIPPPNTFG QAKAPPPKQD241FTQGATKNTN QNSSAHPPHL NMDDTVNQSN IELKNVNRNN AVNQENSRSS STEATNNNPA301NGTQNKPRQP RGAADACTTE KSNKSSLHPN RHGHSSSDPV YPCGICTNEV NDDQDAILCE361ASCQKWFHRI CTGMTETAYG LLTAEASAVW GCDTCMADKD VQLMRTRETF GPSAVGSDATRTRPLEQKLISEEDLAANDILDYKDDDDKV
419 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.