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PYGM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PYGM Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PYGM antibodies →
$725.00each
Catalog Number: LS-G128349-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PYGM (NM_001164716), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPMM | Myophosphorylase | PYGM | Phosphorylase | glycogen muscle
Origin Species
Human
NCBI GenBank
NM_005609
SwissProt
P11217

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRPLSDQEK RKQISVRGLA GVENVTELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV
61RDHLVGRWIR TQQHYYEKDP KKISGGWQME EADDWLRYGN PWEKARPEFT LPVHFYGHVE
121HTSQGAKWVD TQVVLAMPYD TPVPGYRNNV VNTMRLWSAK APNDFNLKDF NVGGYIQAVL
181DRNLAENISR VLYPNDNFFE GKELRLKQEY FVVAATLQDI IRRFKSSKFG CRDPVRTNFD
241AFPDKVAIQL NDTHPSLAIP ELMRILVDLE RMDWDKAWDV TVRTCAYTNH TVLPEALERW
301PVHLLETLLP RHLQIIYEIN QRFLNRVAAA FPGDVDRLRR MSLVEEGAVK RINMAHLCIA
361GSHAVNGVAR IHSEILKKTI FKDFYELEPH KFQNKTNGIT PRRWLVLCNP GLAEVIAERI
421GEDFISDLDQ LRKLLSFVDD EAFIRDVAKV KQENKLKFAA YLEREYKVHI NPNSLFDIQV
481KRIHEYKRQL LNCLHVITLY NRIKREPNKF FVPRTVMIGG KAAPGYHMAK MIIRLVTAIG
541DVVNHDPAVG DRLRVIFLEN YRVSLAEKVI PAADLSEQIS TAGTEASGTG NMKFMLNGAL
601TIGTMDGANV EMAEEAGEEN FFIFGMRVED VDKLDQRGYN AQEYYDRIPE LRQVIEQLSS
661GFFSPKQPDL FKDIVNMLMH HDRFKVFADY EDYIKCQEKV SALYKNPREW TRMVIRNIAT
721SGKFSSDRTI AQYAREIWGV EPSRQRLPAP DEAI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.