Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PWP2H / PWP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116208-100
Catalog Number: LS-G116208
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PWP2H(PWP2) (NM_005049), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EHOC-17 | PWP2H | UTP1 | PWP2
- Origin Species
- Human
- NCBI GenBank
- NM_005049
- SwissProt
- Q15269
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKFAYRFSNL LGTVYRRGNL NFTCNGNSVI SPVGNRVTVF DLKNNKSDTL PLATRYNVKC61VGLSPDGRLA IIVDEGGDAL LVSLVCRSVL HHFHFKGSVH SVSFSPDGRK FVVTKGNIAQ121MYHAPGKKRE FNAFVLDKTY FGPYDETTCI DWTDDSRCFV VGSKDMSTWV FGAERWDNLI181YYALGGHKDA IVACFFESNS LDLYSLSQDG VLCMWQCDTP PEGLRLKPPA GWKADLLQRE241EEEEEEEDQE GDRETTIRGK ATPAEEEKTG KVKYSRLAKY FFNKEGDFNN LTAAAFHKKS301HLLVTGFASG IFHLHELPEF NLIHSLSISD QSIASVAINS SGDWIAFGCS GLGQLLVWEW361QSESYVLKQQ GHFNSMVALA YSPDGQYIVT GGDDGKVKVW NTLSGFCFVT FTEHSSGVTG421VTFTATGYVV VTSSMDGTVR AFDLHRYRNF RTFTSPRPTQ FSCVAVDASG EIVSAGAQDS481FEIFVWSMQT GRLLDVLSGH EGPISGLCFN PMKSVLASAS WDKTVRLWDM FDSWRTKETL541ALTSDALAVT IRPDGAELAV ATLNSQITFW DPENAVQTGS IEGRHDLKTG RKELDKITAK601HAAKGKAFTA LCYSADGHSI LAGGMSKFVC IYHVREQILM KRFEISCNLS LDAMEEFLNR661RKMTEFGNLA LIDQDAGQED GVAIPLPGVR KGDMSSRHFK PEIRVTSLRF SPTGRCWAAT721TTEGLLIYSL DTRVLFDPFE LDTSVTPGRV REALRQQDFT RAILMALRLN ESKLVQEALE781AVPRGEIEVV TSSLPELYVE KVLEFLASSF EVSRHLEFYL LWTHKLLMLH GQKLKSRAGT841LLPVIQFLQK SIQRHLDDLS KLCSWNHYNM QYALAVSKQR GTKRSLDPLG SEEEAEASED901DSLHLLGGGG RDSEEEMLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
919 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.