Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PVRL2 / CD112 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117739-100
Catalog Number: LS-G117739
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nectin 2(PVRL2) (NM_002856), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HVEB | Herpesvirus entry protein B | Poliovirus receptor-like 2 | PVRL2 | Nectin-2 | PRR2 | PVRR2 | CD112 | CD112 antigen | Herpes virus entry mediator B | Herpesvirus entry mediator B | NECTIN2
- Origin Species
- Human
- NCBI GenBank
- NM_002856
- SwissProt
- Q92692
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARAAALLPS RSPPTPLLWP LLLLLLLETG AQDVRVQVLP EVRGQLGGTV ELPCHLLPPV61PGLYISLVTW QRPDAPANHQ NVAAFHPKMG PSFPSPKPGS ERLSFVSAKQ STGQDTEAEL121QDATLALHGL TVEDEGNYTC EFATFPKGSV RGMTWLRVIA KPKNQAEAQK VTFSQDPTTV181ALCISKEGRP PARISWLSSL DWEAKETQVS GTLAGTVTVT SRFTLVPSGR ADGVTVTCKV241EHESFEEPAL IPVTLSVRYP PEVSISGYDD NWYLGRTDAT LSCDVRSNPE PTGYDWSTTS301GTFPTSAVAQ GSQLVIHAVD SLFNTTFVCT VTNAVGMGRA EQVIFVRETP RASPRDVGPL361VWGAVGGTLL VLLLLAGGSL AFILLRVRRR RKSPGGAGGG ASGDGGFYDP KAQVLGNGDP421VFWTPVVPGP MEPDGKDEEE EEEEEKAEKG LMLPPPPALE DDMESQLDGS LISRRAVYVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
479 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.