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PVRIG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PVRIG Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PVRIG antibodies →
$504.00each
Catalog Number: LS-G110075-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PVRIG (NM_024070), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf15 | PVRIG | Transmembrane protein PVRIG
Origin Species
Human
NCBI GenBank
BC001129
SwissProt
Q6DKI7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
34.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTEAQVPAL QPPEPGLEGA MGHRTLVLPW VLLTLCVTAG TPEVWVQVRM EATELSSFTI
61RCGFLGSGSI SLVTVSWGGP NGAGGTTLAV LHPERGIRQW APARQARWET QSSISLILEG
121SGASSPCANT TFCCKFASFP EGSWEACGSL PPSSDPGLSA PPTPAPILRA DLAGILGVSG
181VLLFGCVYLL HLLRRHKHRP APRLQPSRTS PQAPRARAWA PSQASQAALH VPYATINTSC
241RPATLDTAHP HGGPSWWASL PTHAAHRPQG PAAWASTPIP ARGSFVSVEN GLYAQAGERP
301PHTGPGLTLF PDPRGPRAME GPLGVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

326 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.