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PUS7L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PUS7L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119269-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUS7L (NM_001098615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434G1415 | PUS7L
Origin Species
Human
NCBI GenBank
NM_031292
SwissProt
Q9H0K6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEDTDYRIR FSSLCFFNDH VGFHGTIKSS PSDFIVIEID EQGQLVNKTI DEPIFKISEI
61QLEPNNFPKK PKLDLQNLSL EDGRNQEVHT LIKYTDGDQN HQSGSEKEDT IVDGTSKCEE
121KADVLSSFLD EKTHELLNNF ACDVREKWLS KTELIGLPPE FSIGRIVDKN QRASLHSAIR
181QKFPFLVTVG KNSEIVVKPN LEYKELCHLV SEEEAFDFFK YLDAKKENSK FTFKPDTNKD
241HRKAVHHFVN KKFGNLVETK SFSKMNCSAG NPNVVVTVRF REKAHKRGKR PLSECQEGKV
301IYTAFTLRKE NLEMFEAIGF LAIKLGVIPS DFSYAGLKDK KAITYQAMVV RKVTPERLKN
361IEKEIEKKRM NVFNIRSVDD SLRLGQLKGN HFDIVIRNLK KQINDSANLR ERIMEAIENV
421KKKGFVNYYG PQRFGKGRKV HTDQIGLALL KNEMMKAIKL FLTPEDLDDP VNRAKKYFLQ
481TEDAKGTLSL MPEFKVRERA LLEALHRFGM TEEGCIQAWF SLPHSMRIFY VHAYTSKIWN
541EAVSYRLETY GARVVQGDLV CLDEDIDDEN FQNSKIHLVT EEEGSANMYA IHQVVLPVLG
601YNIQYPKNKV GQWYHDILSR DGLQTCRFKV PTLKLNIPGC YRQILKHPCN LSYQLMEDHD
661IDVKTKGSHI DETALSLLIS FDLDASCYAT VCLKEIMKHD V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

701 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.