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PUS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PUS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109250-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUS3 (NM_031307), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FKSG32 | Pseudouridylate synthase 3 | PUS3 | TRNA pseudouridine synthase 3 | TRNA-uridine isomerase 3
Origin Species
Human
NCBI GenBank
NM_031307
SwissProt
Q9BZE2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MADNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSAGAGK TKRAFDFSAH
61GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT RLVESRQTSN YHRCGRTDKG
121VSAFGQVISL DLRSQFPRGR DSEDFNVKEE ANAAAEEIRY THILNRVLPP DIRILAWAPV
181EPSFSARFSC LERTYRYFFP RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT
241ILSAQVQLVG QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID
301ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ LWANHAVKTH
361MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS AFVEGVKMRT YKPLMDRPKC
421QGLESRIQHF VRRGRIEHPH LFHEEETKAK RDCNDTLEEE NTNLETPTKR VCVDTEIKSI
481ITRTRPLEQK LISEEDLAAN DILDYKDDDD KV

512 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.