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PUS10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PUS10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106874-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUS10 (NM_144709), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC139 | DOBI | Pseudouridine synthase 10 | Pseudouridylate synthase 10 | TRNA pseudouridylate synthase | TRNA-uridine isomerase | TRNA pseudouridine 55 synthase | PUS10 | Psi55 synthase
Origin Species
Human
NCBI GenBank
AA213749
SwissProt
Q3MIT2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFPLTEENKH VAQLLLNTGT CPRCIFRFCG VDFHAPYKLP YKELLNELQK FLETEKDELI
61LEVMNPPPKK IRLQELEDSI DNLSQNGEGR ISVSHVGSTA SKNSNLNVCN VCLGILQEFC
121EKDFIKKVCQ KVEASGFEFT SLVFSVSFPP QLSVREHAAW LLVKQEMGKQ SLSLGRDDIV
181QLKEAYKWIT HPLFSEELGV PIDGKSLFEV SVVFAHPETV EDCHFLAAIC PDCFKPAKNK
241QSVFTRMAVM KALNKIKEED FLKQFPCPPN SPKAVCAVLE IECAHGAVFV AGRYNKYSRN
301LPQTPWIIDG ERKLESSVEE LISDHLLAVF KAESFNFSSS GREDVDVRTL GNGRPFAIEL
361VNPHRVHFTS QEIKELQQKI NNSSNKIQVR DLQLVTREAI GHMKEGEEEK TKTYSALIWT
421NKAIQKKDIE FLNDIKDLKI DQKTPLRVLH RRPLAVRARV IHFMETQYVD EHHFRLHLKT
481QAGTYIKEFV HGDFGRTKPN IGSLMNVTAD ILELDVESVD VDWPPALDD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

529 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.