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PUF60 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PUF60 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107893-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PUF60 (NM_078480), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FBP-interacting repressor | FBP interacting repressor | PUF60 | RoBPI | Siah-BP1 | Ro-binding protein 1 | RoBP1 | Siah binding protein 1 | FIR | Siah-binding protein 1 | SIAHBP1
Origin Species
Human
NCBI GenBank
NM_014281
SwissProt
Q9UHX1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATATIALVN GQQGGGSEPA AAAAVVAAGD KWKPPQGTDS IKMENGQSTA AKLGLPPLTP
61EQQEALQKAK KYAMEQSIKS VLVKQTIAHQ QQQLTNLQMA AVTMGFGDPL SPLQSMAAQR
121QRALAIMCRV YVGSIYYELG EDTIRQAFAP FGPIKSIDMS WDSVTMKHKG FAFVEYEVPE
181AAQLALEQMN SVMLGGRNIK VGRPSNIGQA QPIIDQLAEE ARAFNRIYVA SVHQDLSDDD
241IKSVFEAFGK IKSCTLARDP TTGKHKGYGF IEYEKAQSSQ DAVSSMNLFD LGGQYLRVGK
301AVTPPMPLLT PATPGGLPPA AAVAAAAATA KITAQEAVAG AAVLGTLGTP GLVSPALTLA
361QPLGTLPQAV MAAQAPGVIT GVTPARPPIP VTIPSVGVVN PILASPPTLG LLEPKKEKEE
421EELFPESERP EMLSEQEHMS ISGSSARHMV MQKLLRKQES TVMVLRNMVD PKDIDDDLEG
481EVTEECGKFG AVNRVIIYQE KQGEEEDAEI IVKIFVEFSI ASETHKAIQA LNGRWFAGRK
541VVAEVYDQER FDNSDLSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.