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PTPRR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPRR Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPRR antibodies →
$725.00each
Catalog Number: LS-G117733-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCPTP1(PTPRR) (NM_002849), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ch-1 PTPase | Ch-1PTPase | EC-PTP | ECPTP | PTP-SL | PTPRR | NC-PTPCOM1 | PC12-PTP1 | PCPTP1 | R-PTP-R
Origin Species
Human
NCBI GenBank
NM_002849
SwissProt
Q15256

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.86 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRAVCFPAL CLLLNLHAAG CFSGNNDHFL AINQKKSGKP VFIYKHSQDI EKSLDIAPQK
61IYRHSYHSSS EAQVSKRHQI VNSAFPRPAY DPSLNLLAMD GQDLEVENLP IPAANVIVVT
121LQMDVNKLNI TLLRIFRQGV AAALGLLPQQ VHINRLIGKK NSIELFVSPI NRKTGISDAL
181PSEEVLRSLN INVLHQSLSQ FGITEVSPEK NVLQGQHEAD KIWSKEGFYA VVIFLSIFVI
241IVTCLMILYR LKERFQLSLR QDKEKNQEIH LSPITLQPAL SEAKTVHSMV QPEQAPKVLN
301VVVDPQGRGA PEIRATTATS VCPSPFKMKP IGLQERRGSN VSLTLDMSSL GNIEPFVSIP
361TPREKVAMEY LQSASRILTR SQLRDVVASS HLLQSEFMEI PMNFVDPKEI DIPRHGTKNR
421YKTILPNPLS RVCLRPKNVT DSLSTYINAN YIRGYSGKEK AFIATQGPMI NTVDDFWQMV
481WQEDSPVIVM ITKLKEKNEK CVLYWPEKRG IYGKVEVLVI SVNECDNYTI RNLVLKQGSH
541TQHVKHYWYT SWPDHKTPDS AQPLLQLMLD VEEDRLASQG RGPVVVHCSA GIGRTGCFIA
601TSIGCQQLKE EGVVDALSIV CQLRMDRGGM VQTSEQYEFV HHALCLYESR LSAETVQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

657 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.