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PTPRO Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTPRO Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTPRO antibodies →
$504.00each
Catalog Number: LS-G109429-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLEPP1(PTPRO) (NM_030669), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLEPP1 | PTP phi | PTP-U2 | PTPRO | PTPU2 | PTPase U2 | R-PTP-O | NPHS6 | Phosphotyrosine phosphatase U2 | PTPROT | PTP-OC
Origin Species
Human
NCBI GenBank
NM_002848
SwissProt
Q16827

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTEMNPNVV VISVLAILST LLIGLLLVTL IILRKKHLQM ARECGAGTFV NFASLERDGK
61LPYNWRRSIF AFLTLLPSCL WTDYLLAFYI NPWSKNGLKK RKLTNPVQLD DFDAYIKDMA
121KDSDYKFSLQ FEELKLIGLD IPHFAADLPL NRCKNRYTNI LPYDFSRVRL VSMNEEEGAD
181YINANYIPGY NSPQEYIATQ GPLPETRNDF WKMVLQQKSQ IIVMLTQCNE KRRVKCDHYW
241PFTEEPIAYG DITVEMISEE EQDDWACRHF RINYADEMQD VMHFNYTAWP DHGVPTANAA
301ESILQFVHMV RQQATKSKGP MIIHCSAGVG RTGTFIALDR LLQHIRDHEF VDILGLVSEM
361RSYRMSMVQT EEQYIFIHQC VQLMWMKKKQ QFCISDVIYE NVSKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

405 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.